Review-ready finding
THRAP3 — structured candidate finding
Claim
THRAP3 passed all registered source-QC gates in Rest, 8 h stimulation, and 48 h stimulation and ranked #56 by its weakest condition priority score. It is a reproducible source-QC candidate for independent follow-up, not an experimentally validated target.
Supporting evidence
- Rest: rank #7, priority score 0.889, minimum donor concordance 0.620, and guide concordance 0.936.
- 48 h stimulation: rank #130, priority score 0.651, minimum donor concordance 0.503, and guide concordance 0.831.
- 8 h stimulation: rank #152, priority score 0.625, minimum donor concordance 0.383, and guide concordance 0.773.
- Cross-condition maximin summary: rank #56, minimum score 0.625, with 8 h stimulation as the lowest-scoring condition.
Limitations
- The priority score is a relative triage heuristic, not a probability, p-value, or effect size.
- Downstream discovery count is power-sensitive, and donor/guide concordance values were reported by the source authors.
- Condition rank differences are descriptive because this workflow did not fit a perturbation-by-condition interaction model.
Evidence gap
No matching measurement appears in the selected author-provided IL-10/IL-21 follow-up table. Independent replication, orthogonal perturbation, formal endpoint testing, mechanism, safety, and therapeutic utility remain unestablished.
Recommended next experiment
Run an independent multi-donor CRISPRi replication for THRAP3 with a prespecified functional endpoint in Rest, 8 h, and 48 h conditions; fit a formal perturbation-by-condition interaction before making a condition-specific claim, then confirm with an orthogonal perturbation.