Review-ready finding
PLCG1 — structured candidate finding
Claim
PLCG1 passed all registered source-QC gates in 8 h stimulation, 48 h stimulation. It is a condition-specific source-QC candidate for independent follow-up, not an experimentally validated target.
Supporting evidence
- 8 h stimulation: rank #4, priority score 0.901, minimum donor concordance 0.688, and guide concordance 0.822.
- 48 h stimulation: rank #36, priority score 0.822, minimum donor concordance 0.590, and guide concordance 0.758.
Limitations
- The priority score is a relative triage heuristic, not a probability, p-value, or effect size.
- Downstream discovery count is power-sensitive, and donor/guide concordance values were reported by the source authors.
- Condition rank differences are descriptive because this workflow did not fit a perturbation-by-condition interaction model.
Evidence gap
No matching measurement appears in the selected author-provided IL-10/IL-21 follow-up table. Independent replication, orthogonal perturbation, formal endpoint testing, mechanism, safety, and therapeutic utility remain unestablished.
Recommended next experiment
Run an independent multi-donor CRISPRi replication for PLCG1 in the eligible condition set with a prespecified functional endpoint, then confirm the result with an orthogonal perturbation and targeted mechanism assay.